Part:BBa_K2406079:Design

Lox-Term-Rox Measurement Construct

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 886
  Illegal AgeI site found at 998
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The overhangs of our primers each had one half of the target site-term-target site construct.

Source

We made this part by using special primers to add our section to the BBa_J04450 part.